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Detection of higenamine and coclaurine in mice A urine and B feces by LC–MS/MS analysis. The samples were collected at 12, 24, and 48 h after oral administration of authentic coclaurine into BALB/cCrSlc (0.5 mg/mL, 15 mg/kg). Area and IS-area indicate area of sample and internal standard <t>(diazepam-d5),</t> respectively. n.d.: not detected
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Detection of higenamine and coclaurine in mice A urine and B feces by LC–MS/MS analysis. The samples were collected at 12, 24, and 48 h after oral administration of authentic coclaurine into BALB/cCrSlc (0.5 mg/mL, 15 mg/kg). Area and IS-area indicate area of sample and internal standard <t>(diazepam-d5),</t> respectively. n.d.: not detected
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Detection of higenamine and coclaurine in mice A urine and B feces by LC–MS/MS analysis. The samples were collected at 12, 24, and 48 h after oral administration of authentic coclaurine into BALB/cCrSlc (0.5 mg/mL, 15 mg/kg). Area and IS-area indicate area of sample and internal standard <t>(diazepam-d5),</t> respectively. n.d.: not detected
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Detection of higenamine and coclaurine in mice A urine and B feces by LC–MS/MS analysis. The samples were collected at 12, 24, and 48 h after oral administration of authentic coclaurine into BALB/cCrSlc (0.5 mg/mL, 15 mg/kg). Area and IS-area indicate area of sample and internal standard <t>(diazepam-d5),</t> respectively. n.d.: not detected
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Detection of higenamine and coclaurine in mice A urine and B feces by LC–MS/MS analysis. The samples were collected at 12, 24, and 48 h after oral administration of authentic coclaurine into BALB/cCrSlc (0.5 mg/mL, 15 mg/kg). Area and IS-area indicate area of sample and internal standard <t>(diazepam-d5),</t> respectively. n.d.: not detected
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Detection of higenamine and coclaurine in mice A urine and B feces by LC–MS/MS analysis. The samples were collected at 12, 24, and 48 h after oral administration of authentic coclaurine into BALB/cCrSlc (0.5 mg/mL, 15 mg/kg). Area and IS-area indicate area of sample and internal standard <t>(diazepam-d5),</t> respectively. n.d.: not detected
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Detection of higenamine and coclaurine in mice A urine and B feces by LC–MS/MS analysis. The samples were collected at 12, 24, and 48 h after oral administration of authentic coclaurine into BALB/cCrSlc (0.5 mg/mL, 15 mg/kg). Area and IS-area indicate area of sample and internal standard (diazepam-d5), respectively. n.d.: not detected

Journal: Journal of Natural Medicines

Article Title: Metabolism of coclaurine into the WADA-banned substance higenamine: a doping-relevant analytical evaluation of Kampo extracts

doi: 10.1007/s11418-025-01940-4

Figure Lengend Snippet: Detection of higenamine and coclaurine in mice A urine and B feces by LC–MS/MS analysis. The samples were collected at 12, 24, and 48 h after oral administration of authentic coclaurine into BALB/cCrSlc (0.5 mg/mL, 15 mg/kg). Area and IS-area indicate area of sample and internal standard (diazepam-d5), respectively. n.d.: not detected

Article Snippet: Diazepam-d5, provided as the NAGINATA Internal Standard Solution, was purchased from Hayashi Pure Chemical Ind., Ltd. (Osaka, Japan).

Techniques: Liquid Chromatography with Mass Spectroscopy

In vitro metabolisms of coclaurine in A phase I and B phase II reaction by HLM. Coclaurine was incubated with HLM, and metabolites at 0, 1, 3, 6, 12, and 24 h were analyzed by LC–MS/MS based on higenamine, coclaurine, and their glucuronide conjugates. Area and S-Area indicate area of sample and internal standard (diazepam-d5), respectively

Journal: Journal of Natural Medicines

Article Title: Metabolism of coclaurine into the WADA-banned substance higenamine: a doping-relevant analytical evaluation of Kampo extracts

doi: 10.1007/s11418-025-01940-4

Figure Lengend Snippet: In vitro metabolisms of coclaurine in A phase I and B phase II reaction by HLM. Coclaurine was incubated with HLM, and metabolites at 0, 1, 3, 6, 12, and 24 h were analyzed by LC–MS/MS based on higenamine, coclaurine, and their glucuronide conjugates. Area and S-Area indicate area of sample and internal standard (diazepam-d5), respectively

Article Snippet: Diazepam-d5, provided as the NAGINATA Internal Standard Solution, was purchased from Hayashi Pure Chemical Ind., Ltd. (Osaka, Japan).

Techniques: In Vitro, Incubation, Liquid Chromatography with Mass Spectroscopy